In the analysis of significance, how do we group these columns in letter-based style (a, ab, b, c, cd, d ... etc)?.. Is there any easy software to use?
Those letters are often called a compact letter display.
You start with a test that compares the treatments and reports p-values. Usually the test will compare each treatment to each other treatment. As examples, Tukey HSD or Dunn test could be used.
From there, you assign the letters so that any two treatments sharing a letter are not significantly different. This can be done by hand, although in some cases this can get tricky.
As a software solution, the cldList function in the rcompanion package † in R can be used.
In the following example, there are three treatments and three total comparisons. A test such as Tukey or Dunn had been run earlier to determine the p-values.
Thank you very much for your reply and I hope you and all colleagues are doing very well at aec, especially Dr. Araabi.
In fact I solve the problem a while ago with a software called co-stat, I highly appreciate your help and my advice to have a look on that software I used cuz it's also convenient and windows friendly application.