Mazen Alazem

7 Questions 11 Answers 0 Followers

Questions related from Mazen Alazem

How to extract NLR genes from a database?

Greetings to all, I am not a bioinformatics person, but looking for a method that help me extract all NLR genes from benthamiana, tomato and soybean databases.. any suggestions so I can use my...

09 February 2021 4,961 2 View

Measuring small RNA abundance, without sequencing?

Dear all, Do you know any reliable and fast method to measure the levels of plant small RNAs (21,22 and 24 mer)? I am interested in only comparing the abundance of those small RNA between mock...

22 January 2021 4,322 6 View

What is the capacity (insert size) of binary expression vectors?

Hello all, Is there any limit for the insert size in the binnary vectors such as pCambia 1300, pPZP, or pBin61 (used for agroinfiltration)? Big genes (around 4 Kb) are inserted correctly, but not...

04 April 2019 9,964 1 View

Abscisic acid and transcription factors?

Do you know any example where abscisic acid or salicylic acid induce tissue-specific or age-specific transcription factors?

19 September 2018 7,800 2 View

In statistical analysis, how do we group data in subgroups (a, ab, b, c d.. etc) to show the significant differences?

Dear All, I have several charts with ~ 15 columns in each. In the analysis of significance, how do we group these columns in letter-based style (a, ab, b, c, cd, d ... etc)?.. Is there any easy...

19 August 2018 3,462 9 View

Do you know any server/software that can predict structure of large proteins (>150 kDa)?

Available severs allowed an input less than 1000 a.a., but my protein is rather large (~ 1500 a.a) , it is CC-NBR-LRR gene that I would like to predict its structure. Your suggestions are highly...

12 June 2018 9,943 1 View

Do you think extracting protoplast from plants will significantly affect the content of abscisic acid and other injury-related hormones?

Protoplast extraction, plant hormones, mechanical injury induced hormones. Assays on cellular levels.

13 November 2014 2,449 3 View