In estimation of total phenol and flavnoids per eg.
If Conc . 1000 (mg/l) Abs 1.2
Then If conc. 500(mg/l) Abs. 0.6? Is that compulsory?
If the concentration is half then Absorbance should be half?
In many research articles it does not follow this trend.. There is decrements in absorbance ,but it is not directly proportional.
So how should be the actual curve for total phenols and flavnoids
if you are following proper method and suitable wavelength, your absorbance is always proportional to your concentration.you should prepare lower concentration standards like 10ppm,20 ppm,30 ppm,40ppm and 50 ppm, dilute your sample as per required, keep your uv-vis absorbance< 1.0 AU.
According to Beer-Lamberts law, Absorbance is proportional to concentration. However, this needs to be proven scientifically by taking the absorbance readings of various dilute concentrations and producing a standard curve. This curve helps show you the R-square value which tells you the degree of linearity. Different solvents behaves differently. Maintaining a high degree of precision is important for the generation of standard equation with corrected error.
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