Lopa Pattanaik

23 Questions 40 Answers 0 Followers

Questions related from Lopa Pattanaik

What is the best method to recover and quantify ethanol from glucose fermentation media using yeast culture (without HPLC/ GC)?

I am using a fermentation media of composition mentioned below and yeast (S. cerevisiae) culture •3g/L yeast extract •43g/L reducing sugars(glucose), •3g/L peptone, •1g/L KH2PO4 •0.5g/L...

07 September 2021 5,479 11 View

What is the key reactions involved in heterotrophic nitrification and aerobic denitrification? What is the Gibbs free energy?

Kindly provide detailed reactions involved in heterotrophic nitrification and aerobic denitrification and how to calculate the gibbs energy of these process

15 December 2019 4,739 2 View

Can anybody describes the entire carbon transformation mechanisim (chemical/ biochemical reaction) during composting?

In the composting process, the degradation of organic carbon present in the organic matter leads to the formation of either Co2 or CH4 or both and later generation of volatile fatty acids or low...

30 November 2019 6,008 6 View

What is calorific value of ethanol in MJ/l?

If any one have any publication mentioning calorific value of ethanol in MJ/l?

19 April 2018 5,155 3 View

Can we use MTT assay to determine microbial load in compost samples ?

Instead of spread plate method in different media for determining bacteria and fungus colonies . can we determine overall cell viability (microbial cells as other raw material cells are dead or...

01 October 2017 2,909 3 View

In stand curve of total phenol and flavnoid estimation does the concentration is directly proportional to absorbance?

In estimation of total phenol and flavnoids per eg. If Conc . 1000 (mg/l) Abs 1.2 Then If conc. 500(mg/l) Abs. 0.6? Is that compulsory? If the concentration is half then Absorbance should be...

28 June 2017 2,728 2 View

Does CHN value increases after hydrolysis of biomass?

I did the characterization of the biomass. The CHN value of before and after hydrolysis of the plant biomass was compared.The hydrolyzed biomass shows an increase in C and H, but decrease in N as...

18 November 2016 8,458 3 View

What is the standard protocols for sampling waste water,retaining its nutrient value ?

What is the standard protocol for sampling wastewater from any place?If keeping in cold condition is not in the option and the nutrient value has to be retained.

20 July 2016 6,124 5 View

Can anyone suggest some good protocol for carbohydrate,protein and lipid estimation of plant biomass?

The plant is a legume plant.The plant biomass is a mixture of both leaf and stem(Whole plant biomass) I want a good estimation method for carbohydrate,protein,lipid estimation of this plant...

11 July 2016 9,580 3 View

How to calculate manually LCA(Life cycle assessment )of plant based products?

What is the step wise procedure for manual calculation of LCA for a specific plant based products. The system boundary includes: 1. Ploughing 2. fertilizer and organic manure...

04 May 2016 9,535 10 View

How can you select the biomass for its potential use for bioethanol/biofuel/biogas?

What is the selection criteria for any biomass to determine for which thing it can be used? If I want to use any biomass for bio-ethanol production or biodiesesl or biogas production how do I select?

19 February 2016 10,018 8 View

Can leguminous plant be used for biofuel production?

Is there any report of leguminous plants used for biofuel such as bio-ethanol production?

15 February 2016 5,097 5 View

How do I isolate crude enzymes from bacterial and fungal cultures?

I want to Isolate /extract crude enzymes containing beta glucosidase and others? I want a standardised protocol for this

17 November 2015 4,010 6 View

How does a compound detected in Flurosence spectroscopy can be detected in HPLC?

I am detecting my compound named indoxyl (which is highly unstable) in flurosence spectrophotometer using a standard indoxyl acetate. I want to measure the same compound in HPLC -PDA .One reported...

30 October 2015 5,807 0 View

How do I determine a reaction is mass transfer dependent or kinetics dependent?

If in a batch reactor both aeration and agitation is provided, but due to change in agitation there is not significant increase in product yield, but due to aeration and sparging there is...

19 September 2015 7,692 3 View

If dissolved oxygen in the system is not increasing due to agitation ,is that the limitation of agitator design?

I am providing oxygen through sparging to my system (fermented broth) and it increases drastically the DO level in the broth. I am doing also agitation in the system for breaking of the large...

23 June 2015 3,721 16 View

Is glucose released from plant biomass if we soak it in water?

My plant biomass contain a secondary metabolite indican, that get hydrolysed when I soak the plant biomass in water. Now there is two product that I can get from this hydrolysis. 1. glucose 2. An...

12 June 2015 8,224 15 View

Why is oxidation of organic compound enhanced or carried out at alkaline pH?

With respect to oxygen molecule oxidation how alkaline pH helps in fast oxidation?

08 May 2015 9,551 10 View

How can we find out the kinetics of product yield vs time?

To find out kinetics of a reaction always conc. and time is needed. Is there any concept of finding out kinetics of product yield with respect to time?

08 April 2015 1,267 6 View

How can I measure concentration of gaseous oxygen?

Suppose air is coming out of a reactor system. In which our target is to measure only gaseous oxygen that is coming out of the system. What are the easy, convenient and reliable methods to measure...

27 March 2015 6,718 4 View

How do I determine rate of reaction or kinetics of reaction without taking into consideration the reactant concentration?

can we find out rate of reaction only on product concentration

24 March 2015 329 20 View

How can I perform scale-up of a batch reactor with agitation and aeration system attached?

The reactor volume in small scale is 2 ltr., has agitator and sparger .What are the steps to be followed to scale-up the reactor ,so that I can get the product in 10 ltr.in higher amount(~10...

02 March 2015 9,667 5 View

How can I determine the kinetics of aeration agitation and oxidation duration of a non-biological fermentation?

The reactor is a batch reactor having agitator and sparger system attached.I am working on Indigo dye formation without involvement of any micro-organism .The product formation is merely a...

02 March 2015 9,859 14 View