Hello,

I have been recently analyzing targeted exome sequencing data and have been using in silico tools such as SIFT, PolyPhen-2 etc for variant prediction. However, I am finding it difficult to analyze novel variants as well as splice site variant

I have 2 main queries

1. Which in silico tools to be used for predicting novel frameshift variants?

2. Which in silico tools to be used for splice site variant prediction?

Any help or guidance will be greatly appreciated.

Thanks