How do you co-culture astrocytes and neurons?

I am planning to co-culture primary astrocytes with immortalized neurons GT1-7 (both from mice). What is the best way to do this?

05 November 2020 5,040 4 View

HOW CAN I DO AWAY WITH ERROR TERM HAVING ZERO DEGREE OF FREEDOM SO THAT TUKEY POST HOC TEST CAN BE PERFORMED.

04 May 2017 3,828 2 View

Can anyone point me to the best papers describing the format and use of 'User Stories' during requirements analysis/engineering?

Can anyone point me to the best papers describing the format and use of 'User Stories' during requirements analysis/engineering?

24 November 2015 4,448 3 View

What statistical analysis would be most appropriate for this (Forensic Entomology) data?

I have data from a 'crime scene' with species and number of individuals from both a control patch of soil and from a shallow grave. I am looking to see if there is a significant difference in the...

12 November 2015 6,962 2 View

What statistical analysis would be most appropriate for this (Forensic Entomology) data?

I have data from a 'crime scene' with species and number of individuals from both a control patch of soil and from a shallow grave. I am looking to see if there is a significant difference in the...

10 November 2015 4,228 4 View

Anyone familiar with abaqus Shell Elemet Stress Recovery?

07 May 2015 9,036 1 View

Is there a large animal model for pancreatic adenocarcinoma?

I am looking to use arterial caterterization for PDAC treatment.

23 December 2013 5,318 2 View

Can anyone explain Upton and Brooks change model?

Seeking better understanding of the model

11 December 2013 2,490 0 View

What software do you use to write and organize fieldnotes?

(Specifically for Mac software)

03 May 2013 7,170 19 View

How do I view the symbology for 4 band raster imagery in ArcMap? Is the alpha symbology understood as band 4 and does not appear in the TOC?

This is for NAIP imagery of California from 2009.

13 December 2012 3,287 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View