Met-Xaa-Xaa-Arg sequence?

03 April 2016 4,158 4 View

No title

08 September 2015 9,138 5 View

Can anyone recommend a software tool that will create a mass spectra of the theoretical library of peptides/peptidomimetic?

Morning everyone l am looking for software tool that will create a mass spectra of the theoretical library of peptides/peptidomemtic. Any suggestions advice. We need to verify the experimental...

06 July 2015 2,550 2 View

Would anyone like to collaborate in the synthesis of the amino acids shown below?

04 May 2015 8,739 6 View

Any suggestions on how to deal with these two sets of data: One healthy with only zero values and a second set of sick with broad distribution?

statistical data interpretation

01 February 2015 352 8 View

Does anyone has an idea how small particles (subcell dimension) can be separate on cell sorter? Is it for example range of 100 nm or 10 nm?

Looking for method that allowed to separate organelles that are between 50 nm and 5 nm. Any suggestions?

11 December 2013 4,303 4 View

Can anyone recommended a protocol to lyse the cell and later on follow the proteinase activity?

Proteinase activity and whole lysate protocol question. Does anyone What about detergent and reducing agents?

08 September 2013 8,883 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View