13 August 2025 1 10K Report

In patients with rheumatoid arthritis who have contraindications to methotrexate, what evidence-based alternative treatment options are currently recommended, and how do their long-term efficacy and safety profiles compare?

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What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

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What are the most PROBLEMS of HYBRID nanofluid in oil industry ?

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What are the current challenges and future prospects of integrating artificial intelligence into recognition systems for autonomous vehicles?

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How can artificial intelligence be leveraged to enhance the accuracy and efficiency of climate change impact predictions on agricultural productivity?

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Who wants opportunities for scientific cooperation?

Dear Colleagues, I hope this message finds you well. My name is Noor Al-Huda K. Hussein,and I am a researcher specializing in deep learning applications in genetic data analysis. I am currently...

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Dear Colleagues, I hope this message finds you well. My name is Noor Al-Huda K. Hussein, and I am a researcher specializing in deep learning applications in genetic data analysis. I am currently...

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Can we find the Gromacs version from XTC file or TRR?

Hello everyone, This soudns like a strange question, but I created five XTC files using five different versions of GROMACS some years ago. All other files are missing, and I want to repeat the...

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Does sodium level of blood in rheumatoid arthritis patient using methotrexate as DEMARDS falls from the normal value?

It is recently reported as a case study in a medical journal that Sodium level in blood falls down to 119 mEq/L leading to hyponatrenia. Will anybody suggest if it is so what precautions can be...

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What should we do to resolve the problem of formation of metabolites and disintegration of drug during drug release application of hydrogel ?

We are working on polysaccharide based hydrogel (containing high vitamin C), on its drug (Paracetamol also we used Methotrexate salt) release studies using phosphate buffer as a solvent it is...

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How can I induce hepatic damage in Wistar Albino rats by means of Methotrexate?

I tried to induce hepatic damage in Wistar Albino rats by using Methotrexate (IP injection) two doses each one 20mg/kg, Unfortunately it did not work, and the livers of the rats remained healthy.

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In case of invitro drug study on cell lines can I use powder made from available tablets or I need to buy powdered drugs from companies like sigma?

I have read many research papers where they had used drugs like erlotinib,gefitinib,sorafenib,dasatinib,methotrexate etc but I am confused about their source.There are tablets available for drugs...

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How do you do methotrexate selection of Arabidopsis transformants?

So I have always used Hygromycin/Kanamycin selection for Arabidopsis but this is my first time I am using methotrexate for selection of T0 transformed plants. Plants with hygromycin resistance I...

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Stability of doxorubicin and/or methotrexate in RPMI?

Hello, I diluted stock solution of both MTX and doxo in RPMI today (separately) and was hoping to use the rest of the dilution tomorrow for some Jurkat cells (less than 24 hours after dilution),...

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DHFR-MTX selection in CHO (DHFR -ve cells ) ?

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DHFR-MTX selection in CHO-S cell line ?

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I need help with MCF-7 cell cycle synchronisation?

I need help with a cell cycle synchronisation protocol for MCF-7 cells. I have tried the following methods: 1) 0% serum starvation for 24 hours, b) 0% serum starvation for 32 hours, c) 0% serum...

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How to calculate the sample size required to detect the difference in pharmacokinetics of methotrexate?

I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...

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