In HPLC/UPLC/LC-MS etc., some methods are developed (involving buffers) in order to analyze a particular sample. My compound is soluble only in DMF and DMSO. Is there any method which I could try for my compounds?
Hi Mala, you can try to prepare a mother solution of your compound in DMSO, then dilute a portion of your mother solution, let's say 10 uL, in 200 uL of 80/20 MeOH/H2O or ACN/H2O. With the new solutions you should be able to perfom an ESI-mass analysis.
I thought of the same answer as given by M.L. Capobianco. The samples present in DMF or DMSO can be diluted using methanol or acetonitrile or a combination of methanol-water or acetonitrile-water. I think, the composition of DMSO or DMF in that mixture can be about 5 - 10%.
Make sure that your mobile phase is appropriate else you will have issues with carryover when using DMF/DMSO.
Rohit, do you have faced any problems similar to this. which mobile phase will be the suitable one
Hi Mala, some good suggestions already made. I face similar problems and usually make the sample up in DMSO and add 10 - 20 % methanol. The reason for this is that DMSO freezes in my auto sampler at 8 degrees Celsius and the methanol prevents that. Adding water to the mixture sometimes causes turbidity and compounds becoming insoluble in the DMSO/methanol/water mix. As a starting eluent I will usually increase the amount of organic (methanol or acetonitrile) to 10 - 30% (v/v) but this will depend on your stationary phase as well as the type of compound(s) analyzed. Let us know how you progressed!
Absolutely true. When some compounds don't soluble in ACN or Methanol. I add few amount of DMF ( ~100uL) and add 900 uL of Methanol/ACN ( to prepare 1000ppm solution). It works well with ESI-MS also
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