We plan on creating M9 Minimal Media with the following reagents:
- sodium phosphate (Na2HPO4)
- potassium phosphate (KH2PO4)
- ammonium chloride (NH4Cl)
- sodium chloride (NaCl)
- magnesium sulfate (MgSO4)
- calcium chloride (CaCl2)
Is this recipe already complete, or we still need to add a micronutrient cocktail? or any other reagent?
We plan to use this to culture bacteria where in we will use PET plastic as our carbon source.