What is your suggested method and materials for producing UV-curable transparent coatings?

What are the easy methods for synthesizing UV curable resins? I need to synthesis UV curable coatings. MMA seems to have good features for transparent coatings, but the additives and synthesizing...

24 April 2020 3,256 7 View

What is the command for running comsol in the linux os?

I'm using comsol multiphysics on a cluster that operate linux os. i used this command to run and solve my mph file that named s4: comsol batch inputfile s4.mph outputfile s4solve.mph but the...

25 February 2020 5,691 1 View

How can one learn about the effects of an organic substance on the human body?

For example, we synthesized organic matter called A, at this point we want to know its effects on the human body. I wanted to know if there was a special device for this or were they using other...

16 December 2019 2,223 3 View

How can I attach an amine group (-NH2) to a carboxyl group (-COOH)?

20 September 2019 5,256 5 View

I need a method for plating nano-silicon on a metal substrate?

Coating of metals with silicon

13 August 2019 1,318 3 View

Is it possible to simulate hysteresis effect for piezoelectric material in COMSOL or ansys?

I tried sinusoidal input voltage with the amplitude of 100 V but couldn't get any hysteresis curve

24 May 2019 5,973 4 View

Taqman probe real time PCR...Problem regarding constructing plasmid dna standard curve?

Hi guys......i am facing problems regarding developing standard curves for different copies of plasmid DNA using taqman probe real time PCR. i have successfully obtained standard curve for...

06 March 2018 2,284 3 View

Plasmid DNA copy number dilution?

Hello everybody..... the concentration of my plasmid DNA is 126ng. i want to do real-time PCR taqman probe detection......i want to do serial dilution that should start from 10e8 copy number....so...

21 February 2018 3,230 3 View

Looking for a learning tutorial for TSOL software?

Hello I am trying to find a learning tutorial for TSOL software. I would be thankful if you help me in this way Best Regards Mehran Dehghan

27 October 2017 8,799 3 View

Is there any isoform of Rattus norvegicus TGF beta1 mRNA?

I want to do SYBR real time PCR for relative quantification of TGFB1. Gel electrophoresis of real time PCR product showed two bands in around about 80 bp (79 oC melting peak) and 180 bp (84 oC...

22 July 2017 2,265 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View