In HPLC analysis why is there a shift in Rt value of the same compound?

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In HPLC analysis I am getting a negative dip in chromatogram before peak of compound. Mobile phase is Methanol:water 70:30 +0.01% acetic acid. After washing of column with hot water, the dip...

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Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

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Can you connect an HPLC to a Mass Spec only at a certain time point?

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Baseline drift in HPLC? What causes this?

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How can we use mobile apps for improving students' academic performance?

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Medhat Emad

This link have the same discussion

read answers

https://www.researchgate.net/post/Can_someone_advise_on_why_does_my_HPLC_peak_retention_time_is_drifting_by_05_min_everyday?cp=re68_x_p2&ch=reg&loginT=l_nOKPCL4wneHNQ9EJX_CuhwV0nRLyy2FsYb7c7lHIc%2C&pli=1#view=52719e01cf57d7582d8b47a4

Daniel Ricardo Delgado

Because it is almost impossible to repeat the low analisi exact experimental conditions to the above, since the retention time depends on temperature, mobile phase, column ... among other experimental factors.