How to sort live Polyploid cells for further experiments?

Earlier I used Hoechst stain to sort polyploid cells but problem is after sorting cell does not grow. Seem to me cell does not like growing with Hoechst stain. Do you have any other alternative...

10 November 2017 3,100 4 View

Where does Lentiviral transfer plasmid inserted in transduced cells Especially in Human B cells? Which portion of of Plasmid get inserted and how ?

In this attached plasmid I have two antibiotic resistance gene, can I use either one or any specific one? Why?

09 October 2017 1,712 1 View

How can I get phospho-protein signal in Western Blot?

I am trying to find some phospho protein i.e. Aurora, Btk, Bcl2 etc in WB. Even if I get very strong total protein signal but I failed to see any phospho protein signal!!!! Is there any special...

05 June 2015 9,405 17 View

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02 March 2015 9,020 3 View

How can I transfect suspension cells?

I had tried shRNA Lentiviral particles from Santa Cruz (sc-108083) and siRNA from Life technologies (Lipofectamine RNAiMax Procedure) but failed in both cases. I really appreciate your advice as...

01 February 2015 8,449 10 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View