Shariful Islam

6 Questions 11 Answers 0 Followers

Questions related from Shariful Islam

In DAVID Gene Ontology Functional Annotation Chart what is the Difference among GOTERM_BP vs GOTERM_CC vs GOTERM_MF Term? Also among ALL, DIRECT &FAT?

For clarifications I am attaching the summary of a run

11 November 2017 348 1 View

How to sort live Polyploid cells for further experiments?

Earlier I used Hoechst stain to sort polyploid cells but problem is after sorting cell does not grow. Seem to me cell does not like growing with Hoechst stain. Do you have any other alternative...

11 November 2017 3,125 4 View

Where does Lentiviral transfer plasmid inserted in transduced cells Especially in Human B cells? Which portion of of Plasmid get inserted and how ?

In this attached plasmid I have two antibiotic resistance gene, can I use either one or any specific one? Why?

10 October 2017 1,737 1 View

How can I get phospho-protein signal in Western Blot?

I am trying to find some phospho protein i.e. Aurora, Btk, Bcl2 etc in WB. Even if I get very strong total protein signal but I failed to see any phospho protein signal!!!! Is there any special...

06 June 2015 9,431 17 View

How can I get rid of some small (round shaped, shaky with movement but can’t move away from its position) particles within my cell culture flask?

Hi all, I had something very small, round shaped, motile but not able change position particles within my cell culture (U2932, Granta 519). I have never seen them before and don’t know what it is...

03 March 2015 9,044 3 View

How can I transfect suspension cells?

I had tried shRNA Lentiviral particles from Santa Cruz (sc-108083) and siRNA from Life technologies (Lipofectamine RNAiMax Procedure) but failed in both cases. I really appreciate your advice as...

02 February 2015 8,481 10 View