In Crystal violet assay , how to prepare stop solution?

30 September 2019 0 684 Report

How can I prepare stop solution using (Sodium phosphate monobasic dihydrate) for Crystal violet assay to test cell proliferation ? Do I use distilled water to dissolve the salt, please help.

Badges
Science topic

Similar topics
Papers

More Sanaa Almowallad's questions See All

How can I make a scale bar from a microscope image manually, even when I don't have the metadata of the original image ?

How can I make a scale bar from a microscope image manually, even when I don't have the metadata of the original image?

30 August 2023 1,865 0 View

Source about quality of financial reporting ?

Can you provide me with any information or source regarding how to measure the quality of financial reporting?

08 August 2022 1,473 13 View

Does anyone know about the data sharing policy adopted by most journals?

Respected RG members, Journals have started encouraging share data relevant to the research on public repositories at the time of submission. Does anyone know what data should be shared and...

23 September 2020 634 5 View

To extract or attempt pulpectomy for a second primary molar with questionable progosis in children prior to the eruption of the first permanent molar?

Prior to the eruption of first permanent molars, decisions concerning badly decayed second primary molars can be difficult in the dental office. Which approach do you prefer (extract the second...

12 August 2020 2,856 9 View

Why do most students refuse e-learning even though their use of smartphones is more than anyone?

Why do most students refuse e-learning even though their use of smartphones is more than anyone

17 March 2020 6,961 19 View

Are babies less susceptible to infection with corona virus?

29 February 2020 9,739 16 View

Does anyone know where I can find Support Vector data description SVDDD oode?

Greetings fellow researchers, I am reading about one class classification problem, and from what I've read so far, SVDD sounds more suitable for my data and my problematic. I was wondering if...

31 January 2018 2,194 4 View

Where could i find an algorthm script?

Hello , Could someone tell me where to find an algorithm script for ''optimisation of radio resource management in LTE/LTE -A or 5g, using gam theory Thanks in advance

21 December 2017 2,071 1 View

What is the maximum number of concepts in a Galois Lattice (best upper bound) ?

Intuitively I would say that the number of concepts is bounded by min(2|O| , 2|A|) where O and A are resp the objects and attributes sets. I get this (most likely wrong) intuition from the...

17 February 2017 8,117 1 View

How can one generate topology for meal ion clusters?

i have Fe2S2 cluster linked to two histidines and two cysteines. i tried editing the topology file and i changed the name of HIS, CYS and the cluster to FES. i edited the topology file. but after...

07 August 2016 4,857 3 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View