If the CRISPR/Cas system can recognize several PAM sequences, how can bacteria be transformed?
Because you need a preexisting spacer specific for the transforming DNA for CRISPR/cas to kick in.
Would anyone be able to advise me... I have an RCT with a control and experimental group. Participants were recruited from one school (n=59). Participants were assessed using repeated measures on...
04 August 2024 4,040 6 View
I am interested in publishing an updated version of my final master´s project in Spain and i am not sure which steps to take. I am open to suggestions.
16 July 2024 7,431 1 View
Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...
03 July 2024 3,884 5 View
Please can someone share the list of journals with impact factor published in 2024?
27 June 2024 2,927 0 View
I am working on a paper and need to calculate the q2 value in a model. However, I found that some of the values are negative. I am trying to interpret these negative values. Does anyone have any...
26 June 2024 1,176 0 View
Gostaria de saber se alguém teria este livro em pdf, o DOI ao qual tive acesso não gerou resultados.
11 June 2024 4,042 0 View
I work in glycobiology and one of my duties is synthesize short oligosaccharide with, at least, one sugar universally 13C-labelled. Is there any sequence that can help me with characterization,...
28 May 2024 8,039 0 View
Hi! I left some petri dishes with Pseudomonas aeruginosa cultivated in MHI in the fridge (8°C) during some time off, and when I came back, it was like this. This is just one plate out of many, and...
24 May 2024 5,149 3 View
Estos articulos deben tener estas caracteristicas resumir los siguientes aspectos metodológicos: tipo de enfoque de investigación (cuantitativo, cualitativo o mixto), objetivo, hipótesis, muestra...
15 May 2024 820 0 View
Jump Momentum Force at min Displacement Avg Propulsive Force Braking RFD Avg Braking Force
12 May 2024 768 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...
31 July 2024 7,860 3 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...
23 July 2024 4,925 1 View
Currently I need to calculate detection probabilities (PD) from RCS data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0 Equation. The idea is based on...
22 July 2024 2,851 0 View