How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Which test to use check normality for count data if I have 3 treatments and one dependent variable?

I am working on a experimental design. I have three treatments(three temperatures) . I have 18 fish under each sample and after a desired time I will check if there is any variation in their...

01 January 2021 9,167 3 View

What is the preferable incubation period in injecting ethanol into the duck eggs for antiteratogenic studies?

I have been working on the anti-teratogenic activity of a plant extract towards ethanol induced duck embryos however, many studies use the 14 days incubation period and then remove the embryo for...

17 November 2020 4,701 5 View

How can I get eggs of an univoltine casebearer moth for a threshold study?

I am planning to conduct a cage study to determine a critical density of an univoltine casebearer moth in red clover seed crop. This requires to release first instar larvae in the cages with the...

02 November 2020 6,577 3 View

How can I get help to identify a species of Coniopterygidae from Peru?

I found a species of Coniopterygidae preying on eggs and nymphs of Aleurothrixus floccosus in a lemon tree. I have followed its biology and have pictures of eggs, larvae, pupae and adults. With my...

16 October 2020 6,421 2 View

What is the protocol of eggs sampling from an eggs production unit ?

Dear researchers greetings, I'm working on eggs quality for my Ph.D thesis and I want to know what is the protocol for eggs sampling from a production unit. The main questions I have are the...

13 October 2020 9,716 7 View

Will intron-mediated enhancement of gene expression affect downstream of a fusion protein?

Hello everyone! I'm currently trying to increase the expression of my fusion protein in a C. elegans animal model. After reading papers, I found two possible methods to try: (1) codon-optimization...

29 September 2020 490 2 View

Does FuDR dissolve/dilute into NGM plates when administered to plates?

I am currently trying to use FuDR to run a lifespan experiment using C. elegans. I have a stock solution of FuDR that has been dissolved in water and am trying the determine the concentration of...

27 August 2020 1,674 1 View