Vitor Nolasco Moraes

4 Questions 1 Answers 0 Followers

Questions related from Vitor Nolasco Moraes

How to dilute fatty acids ?

Hi everyone, I need to prepare ngm plates supplemented with fatty acids to grow C. elegans. Thus, I would like to know which solvent can I use to dilute fatty acids (myristic acid and linoleic...

29 September 2021 7,457 4 View

Which method is more eficient to isolate RNA from C. elegans?

I have been trying to isolate RNA from C. elegans by the trizol method, but when I go to the quatification step (with biodrop) the sample has no RNA. I imagine the trouble can be with the lysate...

18 June 2021 6,998 6 View

What is considered a good binding affinity energy value between macromolecules?

Hi everyone, Does exist any threshold for binding affinity value between macromolecules? E.g. Any value lower than -5.0 Kcal/Mol..... How to determine that the binding affinity among different...

23 September 2020 4,467 3 View

In C.elegans longevity assay with FUdR do they lay eggs?

I am trying to perform a C. elegans longevity assay with FUdR, but they are laying eggs. Is it normal? How long the FUdR makes effect in the worms? I mean, how long can I follow the worms without...

29 October 2019 3,216 1 View