Hello everyone,
I recently culture some mouse bone marrow and FACS sorted for MDSC. However, the sort efficiency was low this time (~88%), and I noticed some other types of cell presenting in the culture.
Beside MDSC (round cells), there are multiple larger, spindle/rectangular cells that can be seen in the image. I am suspecting that they are macrophages or fibroblast? I would like to see what others think, and if they affect the result of my T cell suppression assay?
Thanks so much!
Dear Trang!
Most likely, the fusiform cells are fibroblasts.
You also see in this article, please:
Article Primary Marrow-Derived Stromal Cells: Isolation and Manipulation
It is now generally accepted that cells of mesenchymal origin such as the osteoblast, endothelium, fibroblast-like stromal cells, and adipocytes as well as cells of hematopoietic origin such as macrophages, osteoclasts and megakaryocytes functionally contribute to the regulation of the HSPC and its subsequent progeny within the microenvironment (ME).
I would say fibroblasts. Fibroblast are probably one the most non-confusing cells. They maybe one of the cells that have long and thin shapped figure. There are other cells that are as long and shapped, however not many more. I still see there are more cells like macrophages. Could the fibroblast be more clear? Maybe suggest they are mesenchymal cells ?or maybe bone cells (osteoclasts?). I do think fibroblast affect the result of T cell suppression assay. Macrophages affect in a minor way. (Perhaps mesenchymal cells affect in a average way)
Hi all, thank you for your responses!
Alexandr Chernov thanks for sharing the article. Very useful resource.
Mª Angeles Zorrilla Lopez-Perea: I suspected the very thin one are fibroblast as well, but not many of them. These cells all came from HPC, which were simulated for 4 days. They probably all differentiated.
I simulated the HPC with GM-CSF and IL6 prior to sorting. That is probably why there are macrophages. Jodi F Evans Triet Bui
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