I am culturing stem cells on plates coated with gelatin. After finding so many variations of drying time in different protocols, I am wondering what is the reason? In other words, why is it necessary to dry the surface? In the protocol I currently use, some medium is added right after removing the gelatin, would this be not so benefitial for my stem cells?
Any possible explanation/idea would be very welcome.
PS: I think drying the surface could allow proteins to better attach to the plate but this is just a thought...
Thank you
Hi Nerea,
I think, if you air-dry try plates after coating, it just allows you to store them for a longer period of time. You could also use them immediately after coating (i.e. skip the drying step) like in the protocol you are currently using.
I would say, it's only important that the coated surface is dry - however long that takes.
Hi Alexander,
Thanks a lot for your comment and help.
I think you are right since most of the protocols in which air-drying is mentioned, suggest the possibility to freeze those coated plates.
Regards,
Nerea
As you suggested, adhesion to the dish is also better after drying. Adding medium to a wet gelatin gel can result in partial detachment from the dish and surface unevennes.
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