How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Does anyone use the ISE electrodes (ammonia, nitrate etc.) to characterise soils samples and plants extracts? What are advantages & disadvanteges?

I will have numerous soil samples/rice rhizosphere sample to characterise as part of the rice microbiome study. I was told that there are ISE electrodes that can be used to measure ammonia,...

19 July 2020 2,066 3 View

Which anti: GRP94 antibody do you recommend?

I am working with C.elegans strain which expresses H.s. GRP94 protein. I am looking for proper antibody to detect GRP94. Do you have some recommendation or suggestions?

13 September 2016 7,214 1 View

Do I need phylogenetic correction to my analyses when I have in data base 11 species?

Dear all, I am performing different analyses (ANOVA, ANCOVA, correlations etc.) and I am not sure if I need phylogenetic correction. On my dataset I have 11 closely related species from Carabidae...

22 November 2015 5,406 12 View

I'm looking for cell dye, which changes it's colour when entering the cell. Can anyone help?

18 October 2015 6,368 3 View

Does anyone have experience with polytypes of CdI2 and their structural calculations?

Or maybe know someone who has experience in this field?

08 February 2015 4,806 3 View

How can I analyse fluorescent images of Double/triple positive cells?

Hi I am using a Columbus for fluo images analysis. I need to define double or triple positive cells. My nuclei are stained with Hoestch (all cells), Sytox (dead cells only) and FITCs (apoptotic...

05 January 2015 3,830 4 View

Does anyone know how to soften the eyeball for histology (paraffin technique)?

02 December 2014 7,688 3 View

Can anyone help me to improve my Lipase assay Kwon and Rhee?

Could anyone help me to improve my lipase assay. I need to study the lipase activity produced by Bacillus saliphilus isolated from alkaline waste lime. I try to apply the method by Kwon and Rhee....

16 September 2014 9,717 29 View

Does anyone know about an ELISA kit that would measure small amounts of human urocortin 2 (UCN2) in serum/plasma?

Detection range around 10-20pg/ml. I have used one but it didn't even detect UCN2 added to the wells. Thanks.

20 May 2014 6,892 5 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

How to create a graphic with two curve ??

Hello, i want to do a graphic with two curve to compare a toxicity of two metals in spss but i don't know how to do this. Can anyone help

01 February 2021 9,371 7 View