Impact of oceanic plate subduction on the formation volcanic island arcs?

06 July 2023 0 8K Report

What is the impact of oceanic plate subduction on the formation and evolution of volcanic island arcs?

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

Look for qualified candidates of Visiting Scholars to Southwest Jiaotong University?

We invite qualified candidates of Visiting Scholars to engage in research collaboration or deliver lectures on their research, with travel expenses covered. The visiting period is flexible,...

20 July 2024 1,165 1 View

How can we effectively assess and monitor the health of the soft and hard tissues around implants so that problems can be detected and treated early?

Although I have extensive experience in dental implants and periodontal treatment, I recognize that we face some deficiencies in my clinic. First, we need to update our equipment, especially in...

09 July 2024 2,004 3 View

What is the association and mechanism between bacterial diversity in the oral microenvironment and clinical manifestations of periodontal disease?

This research question explores the complex relationship between bacterial diversity in the oral microenvironment and the clinical manifestations of periodontal disease. The oral microenvironment...

09 July 2024 6,210 1 View

The journal change publisher, will my published paper get indexed?

Dear all, May I ask a question about indexed by Scopus. 1 month ago, I have published my paper in the Journal name: Challenges in Sustainability in 20/05/2024. However, this Journal has changed...

06 July 2024 6,734 2 View

How can technology empower EFL teaching?

Scholars from multiple disciplines stressed the importance of AI Technology in ESL teaching from a theoretical perspective. Are there teaching designs that combine theoretical discussions and...

01 July 2024 999 7 View

The difference between the E. Laviron's model and RICHARD S. Nicholson's model for electrode reaction kinetics?

For the measurement of electrode reaction kinetics and electron transfer rate constant, using E. Laviron's model and RICHARD S. Nicholson's model, the greater peak potential separation indicates...

30 June 2024 580 3 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

Have you heard about Technological Surveillance on Oceanographic Research Vessels?

It is important to highlight that technological surveillance on oceanographic vessels is a collaborative effort in which different specialists work together to effectively leverage technology in...

31 July 2024 1,633 0 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

Masonry Interaction Module ABAQUS?

I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...

16 July 2024 5,047 0 View

Are there local (indigenous, native, first nation, aboriginal) definitions of climate anxiety (that do not include western definitions)?

We are writing an essay on climate change and its impact on indigenous people circumpolar north people, native nations, islandic, aborigenal of pacific and so on

16 July 2024 5,248 3 View