Hi is the useful product CD4/CD8 Antibody Cocktail

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1-1:10

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mixed / IgG1

Class

Cocktail

Type

Antibody

Clone

4B12, SP16

Immunogen

CD4-stimulated human leukocytes, CD8-synthetic peptide containing the 13 C-terminal amino acids of the cytoplasmic domain of the a-chain of the CD8 molecule

Conjugate

Unconjugated

Form

Liquid

Purification

purified

Storage buffer

proprietary buffer

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10986753

Product Specific Information

This CD4/CD8 monoclonal cocktail contains Mouse HRP CD4 (Clone 4B12, IgG1 kappa) and Rabbit AP CD8 (Clone SP16, IgG). It is designed for qualitative immunohistochemistry with normal and neoplastic formalin-fixed, paraffin-embedded tissues using the MultiVision anti-mouse/HRP + anti-rabbit/AP detection system (Catalog # TL-012-MHRA). This cocktail is provided at an optimal concentration in an easy-to-use format for visualizing CD4 and CD8 lymphocyte subsets in red and blue, respectively. The use of rabbit monoclonal CD8 allows for superior staining, in both sensitivity and specificity, permiting the use of higher titers compared to mouse monoclonal antibodies. These cocktails are specially formulated from MultiVision polymers that provide increased sensitivity, time-savings, and detection simplicity. The MultiVision AP and HRP polymers are innovative, patented technology. The smaller amino acid polymer subunits minimize conflicts in binding the target protein resulting in more consistent staining and better signal amplification. Ultimately, this gives the user higher sensitivity and antibody efficiency as well as better signal-to-noise ratios. This system is biotin-free, which prevents background staining found with traditional biotin-based methods. The MultiVision polymer detection systems yield high quality double staining in less than 2 hours on formalin-fixed and paraffin-embedded tissue sections. Prior to staining, heat-induced epitope recovery (HIER) is recommended, using 10mM EDTA (pH 8.0) for 20min at 98°C followed by cooling at room temperature for 20min. The kits allow observation of two single-stained cells in red and blue and/or co-localization marked by a purple/brownish mixed-color.

Target Information

CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

I think you may have a problem with the quality of antibodies your using or the condition of staining. You need to consider staining conditions, use different concentrations of your antibodies and a little bit long time.

Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies

https://biocare.net/wp-content/uploads/PDF%20Data%20Sheets/3157DS.pdf

https://www.thermofisher.com/antibody/product/CD4-CD8-Antibody-clone-4B12-SP16-Cocktail/MA5-14432

Article An immunohistochemical study of CD4, CD8, TIA-1 and CD56 sub...