Asma El-Howati

4 Questions 7 Answers 0 Followers

Questions related from Asma El-Howati

Apoptosis assay using flow cytometry, can I use annexin FITC in GFP fluorescent cells?

I have transfected cells with GFP, which emit in close range to FITC and used apoptosis kit that have annexin conjugated with FITC to measure apoptosis and PI to measure death. I am gating for GFP...

08 March 2024 9,656 5 View

Immunohistochemistry for T cells?

Hi, I have been having problems with IHC staining CD4 and CD8 whatever antigen retrieval or modification of the protocols or antibody dilution I used. What's a good antibody for IHC staining CD4...

16 January 2022 3,476 4 View

Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

19 January 2021 7,588 7 View

How to do Standard Curve for cells with MTT or MTS?

I am trying to find the standard curve for my cells. should I incubate my cells for a period of time e,g. 24h, after my serial dilution and before the assay and reading to obtain the optical...

20 September 2019 9,038 4 View