Hello all,
I'm managing a very large scale project, which benefits from many automated processes to produce and image slides stained with a fluorescent conjugate. Surprisingly, the biggest bottleneck is the human labor required to coverslip the slides.
Has anyone explored techniques to prepare slides for viewing using some kind of liquid no-coverslip solution?
I think a coverslip or glass slide is the gold standard for microscopic imaging. I don’t think it's replaced yet. I would appreciate it if any news has come in this regard
If you are dealing with cells Glass bottom 96 well plate for microscopy is your best fried such as those: https://www.cellvis.com/_96-well-glass-bottom-plates_/products_by_category.php?cat_id=11
You can culture, fix, incubate and wash in the same plate and use PBS instead of mounting media.
If your specimens are actual tissue sections you can keep them wet with PBS using a pap pen but I'm not sure it would be significantly less labor than cover slipping the sections.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH