I have a set of forward primers (and a reverse but it s OK) to amplify region in murine Immunoglobulin cDNA. Forwards are for FR1 region of VH.

With which (online) tools i can determine the coverage by these forward  primers of the known immunoglobulin sequences, whether they  cover as much V genes as possible? 

And where can I found database for them? I tried in IMGT, but didn t succeed.

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