I have a set of forward primers (and a reverse but it s OK) to amplify region in murine Immunoglobulin cDNA. Forwards are for FR1 region of VH.
With which (online) tools i can determine the coverage by these forward primers of the known immunoglobulin sequences, whether they cover as much V genes as possible?
And where can I found database for them? I tried in IMGT, but didn t succeed.