26 September 2017 3 206 Report

I am doing the experiment for testing the percentage of immune cells in the peritoneal fluid cells(mouse), i repeated the experiment but each group of data from different mouse were different (the same condition for these mice) and also detect few NK cells and macrophages (i check the normal percentage of NK cells and macrophages should be 7% and 9% in my experiment).I collected the peritoneal fluid by adding 3ml FACS Buffer through Ip injection, cells were centrifuged for 5 minutes (500 G, 4°C), and then added Fc block before staining and staining within 6 hours. I use CD49b for NK cells, CD11b and F4/80 for macrophages, all the antibody is effective. Can you help me to find the problem of my experiment?

More Ya Liu's questions See All
Similar questions and discussions