Ya Liu

6 Questions 11 Answers 0 Followers

Questions related from Ya Liu

Immune cells （NK cells and macrophages）percentage detection by flow cytometer?

I am doing the experiment for testing the percentage of immune cells in the peritoneal fluid cells(mouse), i repeated the experiment but each group of data from different mouse were different (the...

26 September 2017 203 3 View

PMA stimulation and cytokine detection??

I am doing the cytokine expression experiment. I used the PMA (5ng/ml) to stimulate the THP-1(1x106 cells/ml) in the flask. After 48h, I replaced the PMA medium with serum free RPMI1640 medium (no...

15 September 2017 8,574 3 View

Cytokine detection after THP-1 stimulation??

I am doing the cytokine expression experiment. I used the PMA (5ng/ml) to stimulate the THP-1(1x106 cells/ml) in the flask. After 48h, I replaced the PMA medium with serum free RPMI1640 medium (no...

14 September 2017 1,797 4 View

Change the PMA medium with complete growth medium?

I want to use the PMA solution to stimulate THP-1(1x106 cells/ml), and then add LPS to stimulate the cytokine expression. Now, I added the 5ng/ml PMA into the THP-1 medium, I want to ask whether I...

12 September 2017 8,902 2 View

PMA preparation and stimulation?

PMA preparation and stimulation I bought the PMA (1mg) from sigma, and I am ready dissolved it by adding 1ml DMSO (stock solution 1 mg/ml). Then the PMA solution will be divided into 100 tubes...

08 September 2017 8,284 2 View

LPS to stimulate the cytokine expression of THP-1

i add 4 different concentrations of LPS（0ng/ml, 10ng/ml, 100ng/ml and 1000ng/ml）into culture medium of THP-1，i used the Human Inflammatory Cytokine Kit (BD bioscience) to detect the cytokine...

07 August 2017 502 9 View