Hello,
Does anyone have an experience with the immobilization of actin proteins (G- and F-actin) on the mica surface? and also their imaging in aqueous medium (buffer) with QI-mode (modus by Scanning force microscopy)?
Maybe something is wrong with my methode to prepare these proteins, because my results are not reproductive.
If I want to image my sample with QI-mode (quantative Imaging), Which parameters should I considere?
Thanks for helping me.
Lindave K.