I was wondering if pearly gateway is an appropriate vector that can be use for biolistic transformation of YFP fusion construct into onion peel? Has anybody done this before? Could someone please share their protocol? Thanks a bunch in advance.
Hi Anshika, for transient expression you need to have consztructioin whixh is functional in onin epidermis cell. You can made this construction in any vector types. pEarly Gateway should be suitable as well, Good luck!
Thanks a lot for your quick responses. I will be studying mitochondrial localization of my protein into Arabidopsis root and onion epidermal cells. If anyone has any experience/protocols with mitochondrial staining of plant cells with mitotracker, please do share. (None of the protocols in the papers have full details in them). I would really appreciate your help.