I'm trying to measure fluorescent fatty acid uptake by yeast cells with kit. All he available protocols are written for cell lines. Is it impossible?

Evgeniya M Kiseleva @Evgeniya-Kiseleva-2

19 December 2016 0 9K Report

Here there is a kit pritocol

https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/2/mak156bul.pdf

Why there is no data about application of such kind of protocol for yeast?

I cultivated cells in flasks, washed with PBS and inoculated with Dye Loading solution. Result was strange: very low fluorescence level, decreasing (not increasing) in time.

What is wrong?

Thank you in advance!

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