Im interested to perform carrier analysis in duchenne muscular dystrophy cases..but in our setup NGS is not possible...is there any other method ??

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Frederic Lepretre

Hi Anjan,

yes it's possible to screen for mutations in the DMD gene by classic PCR and Sanger, but it will take time and money compared to NGS protocols. send us some more explanation on your NGS issue, maybe we can help.

all the best

fred

Partha PRATIM Das

Yes Anjan Da. You can do the screening using different methods like multiplex ligation-dependent probe amplification (MLPA), RFLP, comparative genomic hybridization (CGH). but the best cost-effective strategy would be qPCR followed by sanger sequencing and monitoring CK level. but that will be time-consuming. Again, as the disease is associated with deletion, duplication and small mutation and SNP, NGS would have been a reliable option.

Anjan Rajkonwar.