Can any one suggest me a simple and effective IHC protocol/methods for evaluation of collagen 1 and 4 in mice dermal tissue samples?
Avoid antigen retrieval, otherwise the non-specific reaction would occur in a deposit manner.
I want to interpritonealy inject resveratrol (10-15 mg/kg) in rats. In most experimental studies resveratrol dissolved in different vehicles such as: ethanol, saline, ethanol+saline, water (pH:6),...
04 May 2016 8,756 10 View
I prepared a manuscript from my research findings at fields of biochemistry and lipid metabolism in laboratory animal study (rat). I need help to improve the level of language writing...
01 February 2015 5,923 3 View
I need experienced pathologists advice about histological assessment of rats liver tissue. In our study we investigated 3 different fatty acids (2 concentrations) in rats for 20 weeks. For...
31 December 2014 5,194 1 View
Histological analysis.
11 December 2014 2,415 3 View
I need some guides or protocols related to the topic , included some parameters such as ; mesangial matrix expansion, glomerulosclerosis, tuft-to-capsule adhesions, hyalionosis( glomerular or...
11 December 2014 9,721 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View