I need some guides or protocols related to the topic , included some parameters such as ; mesangial matrix expansion, glomerulosclerosis, tuft-to-capsule adhesions, hyalionosis( glomerular or tubular) , dilatation of urinary space and thickening basement membrane of bowman's capsule (light microscopy assessment)
Although intended for human applications, this scoring classification system might provide a basic foundation for animal models: Pathologic Classification of Diabetic Nephropathy" in Journal of American Society of Nephrology (2010). Full text at:
http://jasn.asnjournals.org/content/21/4/556.full
It is important to realise, that in most mouse models of diabetic nephropathy their kidneys do not develop the same level of progressive diabetic nephropathy (DN) as observed in patients with DN. So, class 1 and clas 2 DN can develop in most mouse models. Class 3 DN wich includes the development of nodular glomerulosclerosis, is only described in BTBR ob/ob mice that lack both the gene coding for leptin and the leptin receptor.
The level of mesangial sclerosis and hyalinosis can be quantified by Image-J assisted digital image analysis determining the area percentage of mesangial extracellular matrix using PAS-stained paraffin sections.
The level of interstitial fibrosis can be quantified using Sirius red stained paraffin sections.
thanks every one
Dr Emile De Heer, i know nodular sclerosis (Kimmelstiel–Wilson lesions) do not develop in mouse diabetic models , while some previous studies described this stage !! however, i stained paraffin sections of severe diabetic rat kidney with PAS and Masson Trichrome. can i use Image J for tubular fibrosis?
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