If Tm of forward and reverse SSR primers is more than 70 C then what can i do to run the PCR?

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If difference between Tm of forward and reverse SSR primers is more than 10 C and this primer is useful then what to do for getting good result?

My question is that....If difference between Tm (Melting temperature) of forward and reverse SSR primers is more than 10 0C and this primer is useful then what to do for getting good result?

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Andrey Shirak

I don't see any problems or general complications of high Tm

M. A. El-Sayed

Hi,Parmeshwar Sahu,

A.T. of SSR primers not more than 65 C

Parmeshwar Sahu

Hii Hasan Sir,

Why A.T. of SSR primer should not more than 65C ? I am asking about the problems arises due to high A.T. (>65C) ?

Elongation and annealing stages merged and likely you must use in specific Taq polymerase. I use in SuperTerm DNA polymerase