If Tm of forward and reverse SSR primers is more than 70 C then what can i do to run the PCR?

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If difference between Tm of forward and reverse SSR primers is more than 10 C and this primer is useful then what to do for getting good result?

My question is that....If difference between Tm (Melting temperature) of forward and reverse SSR primers is more than 10 0C and this primer is useful then what to do for getting good result?

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How to calculate the annealing temperature of a primer pair?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

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What is the ideal efficiency for a qPCR?

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MiRNA qpcr problem?

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Andrey Shirak

I don't see any problems or general complications of high Tm

M. A. El-Sayed

Hi,Parmeshwar Sahu,

A.T. of SSR primers not more than 65 C

Parmeshwar Sahu

Hii Hasan Sir,

Why A.T. of SSR primer should not more than 65C ? I am asking about the problems arises due to high A.T. (>65C) ?

Elongation and annealing stages merged and likely you must use in specific Taq polymerase. I use in SuperTerm DNA polymerase