Hi,
not sure if this is the answer you're looking for, but I had a microbiology course where we did a small enrichment project. We enriched marine bioluminescent bacteria (some vibrio species, can't remember exactly). What we did was that we found a recipe for artificial sea water, then got a freshly caught fish and placed it into the sea water for a day or two. Then we could see the bioluminescent growth on the fish in a dark room, we scraped it off and grown it in adapted medium. When we got enough growth we clean streaked to separate the species. Basically, this procedure: http://www.splammo.net/bact102/102lumbact.html and it worked fine. Hope this helps!
I have done something similar in order to create a "Star wars light saber" for a lecture.
I took a fresh squid and put it in seawater in a fume hood (important since it starts to smell after a day or so). After a few days, you can see bright dots on the squid skin. These you isolate with a needle and streak onto agar plates with marine agar medium. After another few days (at room temerature), you will have colonies coming from just one bacteria cell on the agar plates. These colonies can again be isolated with a needle and inoculated into liquid marine bacteria growth medium (if you want them in a liquid culture. If you then by any chance want to make the "light saber", you just pour the liquid culture into a glass tube (be sure to leave some air in it, since the bacteria need oxygen to produce ligth) and put rubber stoppers in the end.
Good Luck!
Hi, I am unfortunately aware of any robust methods in this field . I will try to watch for it.
Sorry
FR
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Microorganisms
- Bacteria