The DNA materials for native populations of Juglans regia are needed to clarify the relationship of J. regia and J. sigillata. The samples from Europe (Spain, Portugal, France and Turkey etc.) and from central Asia (Afghanistan, Iran and kyrgyzstan etc.) are prefered. People who help to provide samples will be included as coauthor of the related publication.
Dear jie
In the northern part Pakistan the walnuts belonging to Juglans regia are found both as cultivated fruit trees and as patches of natural forests.
As walnuts are deciduous trees, leaves can only be collected during late spring and secondly how much material, from how many trees and when it will be take.
Regards Habib Ahmad
Vice Chancellor Islamia College University
Peshawar Pakistan
Dear Professor Habib Ahmad,
Thank you very much for your kind response.
Yes, it is not the right season to collect the samples at the moment. The spring is good season to get the silica gel dried material.
For sampling strategy, 20-25 trees are ideally for one population from each site. If it is possible, both the native and the cultivated one are needed, and the distance between two individuals are at least 100 meters apart. One to five (or ca. 5g dry material) fresh and clear new leaves (depend on the size, for mature one, one leave is adequate) were collected, then preserved with silica gel.
For my experience, it had better to find a site along the road, then you can drive to collect along the road, which will save energy to climb up the hill and the tree.
If there is no silica gel, and the weather is dry enough, you could dry it in the air. Otherwise, towel can be used in humidity climate.
Thanks agian,
Best wishes,
Jie
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