I know that mRNA level often do not correlate with protein level. But, if mRNA level is zero, than I think protein can not be anyway. Why we should see it at protein level as well? thanks
Hi there,
Zero doesn't mean no transcription (for instance mRNA half life might be very short), it means the mRNA level is under the threshold of detection. As translation is an amplification step (from one RNA molecule you might get several of the corresponding protein), it is worth checking at the protein level.
Hi there,
Zero doesn't mean no transcription (for instance mRNA half life might be very short), it means the mRNA level is under the threshold of detection. As translation is an amplification step (from one RNA molecule you might get several of the corresponding protein), it is worth checking at the protein level.
Apart from Dominique's very valuabkle comments you should always think about accumulation of protein in the cell. If degradation of the protein is slow, you may still have a lot of activity, even if transcription has stopped for quite some time. I strongly recommend, always to look at both levels, if ever possible.
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