If I wash Lactobacillus cells with LiCl to remove an S-layer protein, are lipoteichoic acids removed from bacterial cells too?

Simone Guglielmetti @Simone-Guglielmetti

06 November 2012 0 212 Report

I am extracting S-layer proteins from L. helveticus by washing cells with LiCl 5M. I was wondering if a significant amount of lipotheicoic acids (LTAs) can be co-extracted. How can I verify if LTAs are present in my preparation?

Badges
Science topic

More Simone Guglielmetti's questions See All

High-resolution land cover map of Central Asia?

Hi! I am looking for an high-resolution land cover map of Central Asia. Otherwise, can you suggest me the best world land cover map and the respective download link? Thank you! Dr. Simone Valeri

06 June 2024 3,860 2 View

Where can a physicist work in the field of biomedical research?

I am trained in Physics, but I spent my PhD doing biomedical research (wet lab). Now, I want to continue my career in biomedical research without losing my background as a physicist. So, what is...

25 May 2024 6,579 3 View

Systematic 'Google' review?

I'm writing a paper review / prospective paper about the use of XR tools in the space training domain - research gaps and future applications/investigations. There are not so many papers about the...

28 February 2024 8,649 2 View

How to fit Stokes components simultaneously ?

Hello ! I would like to characterize experimentally Stokes vector of light passing through a half-waveplate with a polarimeter in order to fit the parameters of the angle and the retardance. Up to...

21 February 2024 7,603 2 View

Let me know about publication progress and it acceptability?

Hello, I am Simone from MU, Ethiopia. My question is concerned to publication, shall I publish full of paper and acceptable in all competition?

11 January 2024 5,732 1 View

Which model can I use to fit ellipsometry measurements from 250 to 1600 nm ?

Hola ! I would like to know if you can suggest me a model to fit data from GaAsN samples between 250 up to 1600 nm.

30 November 2023 1,217 4 View

Why I have different results analyzining the same miRNAs of an other scientific work?

I red a paper on an important journal of cardiology. In this work they found that 8 miRNAs were dysregulated in plasma samples of a study cohor of 1710 participants (Controls + patientes affected...

28 August 2023 7,609 2 View

Why is important to have an 80% cell confluecy for recombinant protein stimulation?

I have to perform a new experiment. I have to stimulate MDA-MB-231 and THP-1 differentiated with a recombinant protein. I red that cells have to reach an 80% cell confluency before stimulation...

19 July 2023 6,872 4 View

How could I choose the right number of cells and the right plate to perform drug test experiment?

I am working with triple negative breast cancer cell line MDA-MB-231. I want to evaluate the effects of a specific human rrecombinant protein on them and to evaluate cytokine production in...

18 July 2023 2,080 5 View

Proof of the Hodge Conjecture?

Dear all, I think I have proven the Hodge conjecture, but it is difficult for me to say if what I did is error-free. I submitted it to various journals obtaining rejections based not on fatal...

28 May 2023 7,361 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View