If I wanna study PPAR relative pathway in intestinal epithelial cell using Caco-2 as model, should I polarize Caco-2 cell?

15 December 2019 0 2K Report

I found polaried Caco-2 cells suitable for transportion research but I can't confirm whether it would be a good choice for studying signal pathway.

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Any relation between resin viscosity（mPa*s）and resin relative molecule weight？why the former is used in manufacture?

https://en.wikipedia.org/wiki/Viscosity

21 July 2024 3,089 4 View

Why not use CD11b staining to evaluate the purity of primary microglia?

I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?

02 July 2024 9,411 2 View

Does mutation in long terminal repeat (LTR) regions of lentiviral plasmid affect gene delivery?

Hi lentivirus experts, I have lentivirus plasmids that carry mutations in the 3' and 5' LTR region. These regions were not exactly designed for easy cloning. Therefore, I wonder if it is possible...

14 April 2024 3,054 2 View

Can you help me about my simple HVDC system in digsilent?

I've been looking at digsilent to see how HVDC affects the control of generators. It's a simple HVDC connection of buses. The static generator is set up as a reference machine, but as you can see...

13 March 2024 4,582 1 View

ORCA ERROR: Cannot open GBW file?

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24 February 2024 1,081 1 View

How to optimize tumor digestion protocol to avoid clumps?

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23 October 2023 3,729 1 View

In my free vortex induced vibration test, why I cannot find the classic phase shift between the vibration and the force measured by a sensor?

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19 September 2023 2,982 0 View

How to dry oleic acid-capped SPION?

To prepare the solution of SPION at a specific concentration, I need to dry the SPION after precipitating them by ethanol. However, I always failed to get the powder form but kind of gel. I wonder...

23 July 2023 9,414 0 View

How to inhibit the strong volatilization of Ga element at high temperature?

Except adding a small amount of oxygen or increasing the pressure of the growth atmosphere, are there any other methods to inhibit the volatilization of Ga to maintain stable crystal growth...

07 March 2023 9,013 2 View

Why is my QuantStudioTM 3 System running more cycles than what I had set it to?

Hello, I have been performing 9 well experiments over this past week and have had no success in running it. The purpose is to amplify my DNA. In the attached file, all the graphs from the...

28 September 2022 1,386 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

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04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

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04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View