I have worked on invivo toxicity of liver n kidney, now i m shifting towards invitro toxicity of mamallian cancer cell(breast cancer). I just wanted to know how to standralize the protocols i already have according to cells viz sample volume... Or number of cells?
Hello,
which assays/protocols are your using? IHC?
Unfortunately, it is not easily possible to use the same protocols for tissue and for cells, since the latter lack the extracellular environment that might have an impact in how you prepare your samples. You should adapt your protocols for the cell lines you are using and test everything carefully before starting your experiments.
Good luck!
cancer cells have a different needs in compare with another one, now What will happen to that if we compare them with normal cells?
Unfortunately it is impossible.
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