I cultured MDA-MB-231cells in L15+ media and after 2 days the growth is still scanty. Do I just change the media or tripsinize the cells and put them in a fresh culture vessel and fresh media?
Ambreen Alamdar
Hi Stella,
There are many things that you need to consider,
What seeding density actually you used.
whats the doubling time for these cell lines.
Hows the color of the medium look like if its with phenol red dye.
how confluent your cells are right now sometimes if cells are more than 60 % confluent and growing slow you can still passage but with higher seeding density but there is no harm in supplement it with fresh media and see how the cell grows.
Hope this helps
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