What research has been done on learning non-Markovian reward functions?

Recently, some work has been done planning and learning in Non-Markovian Decision Processes, that is, decision-making with temporally extended rewards. In these settings, a particular reward is...

06 April 2019 4,890 2 View

Is this contamination in cell culture?

In my human cell line, I've observed masses that look different from the cells. When seen with the naked eye, they appear to be white spots. When seen under a scope, they appear to be dense...

14 August 2018 8,480 4 View

Why are my THP-1 cells dying so fast?

Im working with THP-1. I culture them well, they grow well, they differenciate well. Then i infect them with 1:1 MOI and i treat them for 4 days with antibiotic. At they 4 almost all the cells...

01 February 2018 6,263 0 View

Estimating sample size: can secondary outcome data be used where primary outcome data does not exist?

I'm estimating sample size for a future study using the primary outcome (mean and SD) from a current study. The future study will use a randomized control design with two independent 'arms' (or...

17 January 2018 4,037 1 View

Can anyone spot any possible problems with this approach to diagnosing colorectal caner?

Take a sample from the suspected area, polyp, ulcer etc. Extract RNA from these cells. Convert to cDNA and do real time PCR to determine whether the oncogene VAV3 is over expressed? (VAV3 is...

25 October 2016 9,528 3 View

What is the definition of the POMDP state value function when the reward function is defined as R:S x A x S->Reals?

When the reward function is defined as R(a,s), the value function is defined as max_{a in A} (rho(a,b) + ...), where A is the set of actions, b is the current belief state and rho(a,b) is the...

05 May 2016 9,561 3 View

Why am I getting a low ICC even though agreement is objectively high?

31 January 2016 4,730 3 View

Can anyone recommend free software which is easy to use, for computing Minimum Cross-Entropy?

I'm working with discrete distributions, and i have only one constraint (over the atomic events) on the posterior distribution. In particular, i have a prior distribution P over four atomic events...

25 January 2016 6,708 2 View

How can I derive the order of a LTI system?

16 January 2016 4,836 3 View

Any tips for transfecting neurons with siRNA in primary culture?

We would like to transfect post-mitotic neurons cultured from early human fetal neocortex with siRNA. As a trial experiment,we have tried Dharmafect 1 to introduce siRNA against GADPH and found 5%...

16 March 2015 3,512 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View