Gavin J Clowry

2 Questions 3 Answers 0 Followers

Questions related from Gavin J Clowry

If GFP is expressed under a specific promoter, and then the cell differentiates and this promoter is no longer active, how long can GFP stay in cell?

I'm wondering what the half life of green fluorescent protein is, and how quickly it is degraded and removed in a neuron. IF we express it via a promoter only active in progenitor cells, could we...

28 June 2022 262 1 View

Any tips for transfecting neurons with siRNA in primary culture?

We would like to transfect post-mitotic neurons cultured from early human fetal neocortex with siRNA. As a trial experiment,we have tried Dharmafect 1 to introduce siRNA against GADPH and found 5%...

17 March 2015 3,559 10 View