What could be source of contamination for seeing positive amplification in negative control?

Yesterday, I ran a gradient endpoint PCR with some primers and saw positive amplification for positive and negative for negative amplification. Running the same assay today, show positive...

13 January 2021 5,201 1 View

I have a monoclonal antibodies (purified) of 8mg/ml, Now I want to evaluate ELISA for my serum samples in International ELISA UNITS?

I need to know how to convert micrograms of antibodies into ELISA Units.

21 September 2020 3,286 3 View

Has anybody found better anticancer effect on patient derived primary cancer cells than cell line?

I am testing compounds on the patient's primary cancer cells as well as a cell line. Generally, I see better activity in cell lines compared to primary cells. Surprisingly I am seeing the...

18 March 2020 467 3 View

Is there an alternate for UV Ozone treatment in order to pursue ETL surface modification?

I wish to deposit a perovskite layer over ETL (SnO2) in order to fabricate the n-i-p planar device and could notice the role of UVO treatment prior to deposition. I was wondering if there any...

28 January 2020 3,310 2 View

Is Girvan Newman and Louvain an effective technique to identify rumors in social networks?

Suggest methods to perform clustering and classification in these- 1) Graph structures 2) Twitter networks 3) Rumor Identification 4) Big data networks

03 December 2019 7,452 3 View

What are the factors affecting Wind Energy Production in Denmark?

Hi everyone, I am a Master's student and I am doing my thesis on Wind Energy Production forecast for Denmark and for that I need the data of the Factors affecting the Wind Energy Production in...

06 November 2019 7,535 2 View

What would be the formula for finding the stretch force for expanding an O ring made of elastic ? On what factors the expansion stretch force depends?

I want to expand an O ring made of elastic material and am looking for the mathematical expression (dynamics) on which the stretch force depends. In addition to this, how does it vary in different...

18 March 2019 4,158 3 View

Catalyst and electrode for fuel cell application?

Hello, could anybody help me to get idea about which kind of ionomers, surfactants, additives and solvents except Nafion, iso-propanol, n-propanol, hexanol, methanol, ethanol, water, ePTFE,...

11 November 2018 8,886 7 View

Is there literature on knowledge management in policy innovations?

I am looking for theories/ frameworks on how knowledge is created and managed in a network on the way to an innovation in public services.

22 September 2018 1,624 4 View

How is inter-coder reliability calculated in qualitative research calculated?

If we are doing a Grounded Theory research involving coding of interview transcripts as propounded by Glaser (1978) and Strauss and Corbin (1990), then: 1. Is there a heuristic to decide on the...

23 October 2017 6,494 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View