I am working on immunohistochemical characteristics of the notochord of the catfish and trying to prove the presence of hyaluronic acid.
The hyaluronan is usually detected in histochemistry using the hyaluronan binding protein (HABP) which is commercially available or can be purified from articular cartilage. This protein is not an antibody but binds specifically the hyaluronan chain and could be fluorescinated with different fluorophore. in order to confirm the hyaluronan presence with HABP you could also pre-treat the sample with hyaluronidase (there are several with different charactersistics) and that add HABP, in this case the signal should be abolished by enzymic treatment.
The hyaluronan is usually detected in histochemistry using the hyaluronan binding protein (HABP) which is commercially available or can be purified from articular cartilage. This protein is not an antibody but binds specifically the hyaluronan chain and could be fluorescinated with different fluorophore. in order to confirm the hyaluronan presence with HABP you could also pre-treat the sample with hyaluronidase (there are several with different charactersistics) and that add HABP, in this case the signal should be abolished by enzymic treatment.
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