I would produce and purify CCMV, CPMV and TMV. Which are the best methods?

08 April 2016 1 7K Report

Is it possible to produce the ricombinant protein and assemble it in vitro as virus like particles?

Recombinant protein production is best way. You can select any expression vector. pMAL or pET. I hope you want to express one particular protein for example CP or MP in that case recombinant method is best one.

Badges
Science topic

More Paolo Passaretti's questions See All

Are there local (indigenous, native, first nation, aboriginal) definitions of climate anxiety (that do not include western definitions)?

We are writing an essay on climate change and its impact on indigenous people circumpolar north people, native nations, islandic, aborigenal of pacific and so on

16 July 2024 5,248 3 View

How can I add a new journal?

I would like to add "RIvista di Agronomia ISSN 0035-6034 to the RG journal list

06 July 2024 7,380 1 View

Could you provide me with the names of journals, in Spanish or English, to make my publication with that journal?

Hello to all members of this community. I am interested in making a publication in a scientific journal about the research I have done, the title of my research is "Project Management Model to...

16 April 2024 6,800 3 View

PVA hydrogel preparation?

Hello everyone, for my master’s thesis I have to make a phantom in PVA. From research in existing literature, the PVA at my disposal is poor for my ultimate purpose (I have thus activated myself...

14 April 2024 7,855 1 View

Trap cameras: any advice/recomendation on rechargeable batteries?

Hi there, I'm setting a monitoring effort based on trap-cameras. I know that the selection of the batteries depends on many factors suh as camera-model, replacement possibilities, etc. and that...

17 November 2023 4,333 1 View

Is it possible to attach a paper to a discussion thread? What is the procedure ?

The previous question is not a file to be added by clicking the bottom "ADD"

30 August 2023 8,658 0 View

Is there a way to extract the velocity arrows from HEC-RAS?

Hello everybody! Is there a way to extract the velocity arrows from HEC-RAS? Or reproduce them externally from the simulation results? Thanks for your kind and professional support

13 July 2023 5,629 2 View

How to find if and citations of my articles?

how to find if and citations of my articles?

10 May 2023 1,831 4 View

Are OPLS-AA Force Field parameters dependent on simulation Temperature?

Dear all, I am evaluating the Young's modulus of graphene via Molecular Dynamics simulation with OPLS-AA potential. The method I am using is a tensile test by "pulling apart the opposite rows of...

16 April 2023 7,788 1 View

Does anyone have experience in mouse pb slides preparation and staining?

I'm currently working with mouse peripheral blood, but several issues came out every time. Actually we are using as collection site the tail, blood is drip in eppendorf containing EDTA and...

12 March 2023 544 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View