By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

Should studies published in predatory journals be included in systematc reviews?

I am conducting a systematic review of intervention, and i observed that one included study could determine the statistical significance of the final meta-analysis. Problem is, that article has...

13 April 2020 922 9 View

OCR system for phonetic (IPA) symbols?

I need to convert manually annotated IPA transcriptions into digital format. Does anybody know of an OCR system able to recognise IPA symbols (even just for English phonemes)?

25 March 2020 5,982 0 View

Is it possible to read the deformation field file produced by SPM during normalization?

If (x,y,z) are the voxel coordinates of a point of the brain scan before normalization, what is its position after applying the deformation field generated during the normalization?

19 February 2020 10,004 3 View

Bibliography on the effect of soils on nectar and sap?

10 September 2019 8,922 4 View

What is the correct phenol red permeability through Caco2 monolayer?

Dear all, I am performing Caco2 permeability experiments using transwell inserts and I am using phenol red permeability assay to verify the monolayer integrity. What is the maximum phenol red...

06 March 2019 6,726 6 View

How much solvent (ethanol) would I need to extract capsaicin (and other capsaicinoids) from siling haba?

Siling haba is a variation of Capsicum annuum var longum. The capsaicin extracted will be tested against aphids to test its insecticidal properties. Someone suggested that I should use 5ml/g or 2g...

12 February 2019 1,373 3 View

How much ethanol do I need in extracting capsaicin from red bell peppers?

The capsaicin recovered will be tested against aphids.

04 February 2019 5,811 5 View

Is anyone aware of studies concerning geostatisics in the environmental field?

I'm looking for studies on environmental analysis carried out by geostatistics

16 January 2019 5,356 4 View

CVD adhesion on silicon substrates?

Dear all, I am currently trying to uniformly deposit organic semiconductor via thermal CVD at low pressure (around 10 Torr), but I sometimes get either no deposition or not homogeneous deposition...

21 October 2018 3,185 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View