How to get rid of the free GFP signal in an Atg8-GFP tagged yeast saccharomyces cerevisiae?

I am working with yeast saccharomyces cerevisiae -BY4741- strain. I tagged the Atg8 with GFP in this background and whenever I am performing western blot I get a free GFP signal for my WT which...

15 November 2020 8,556 3 View

Hi all! I need to activate CD8 T cells extracted from mice spleen in vitro, which option is the best? Concanavalin A? LPS? anti-CD3 and anti-CD8?

in vitro mice CD8 cell activation. These cells are extracted from B6 mice spleen by magnetic separation.

01 November 2020 3,276 2 View

Hi all! What vehicle do you recommend for IP injection in mice for a DMSO soluble drug?

I have 2 drugs (PTC-209 and GSK343) that are soluble in DMSO (I already made the stock solution, dissolved to a clear solution) and I have to inject them IP to mice for a 2 week treatment course....

17 September 2020 9,107 3 View

How reliable/effective "Covidence" for systematic reviews? And is it value for money?

https://www.covidence.org/home

14 July 2020 8,296 2 View

Why is Quantum-dot Cellular Automaton (QCA) technology fail to flourish? Specially, Semiconductor and Magnetic QCA for electronic applications?

In 1993, Lent et al. introduced one of the most promising and emerging computational paradigm known as Quantum-dot Cellular Automaton (QCA) computing. Now, it is almost 30 years have been passed,...

06 May 2020 4,955 7 View

Is it possible to have more elements than nodes in mesh analysis of ansys?

During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?

04 May 2020 4,700 11 View

Which are the best journals which accept newcomer students for writing reviews on topics related to biomedical engineering ?

I am looking to communicate with a personnel who deals with beginner-level review articles, and want to learn how to write good reviews and the rules and regulations to follow.

06 March 2020 6,068 3 View

In which reason EDTA used in injectables containing proton pomp inhibitors?

We know that EDTA is used by different reasons (chelating agent, support for antimicrobial effectiveness e.g) in pharmaceuticals. Is there any specific reason to prefer EDTA in injectables...

01 December 2019 5,166 3 View

How to get mean age of fluid for a closed system using FLUENT?

Hi everyone, I am working on simulation of stirred tank reactor operating at batch mode. So, there is no fluid inlet-outlet in the system. Can I calculate the local mean age of fluid by using...

18 November 2019 1,142 3 View

How is the length of any scientific document (report, thesis, essay, conference paper, journal article) calculated ?

I wish to know what aspects of these technical documents are included and excluded while making a word count. Specifically, say, a figure caption, references, tables, table footnotes, page...

24 June 2019 9,815 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View