I have tibilias muscles frozen in nitrogen prior to put them at -80
Do you want to measure it by biochemical methods or by fluorescence methods ?
By biochemical methods I think HPLC or LC/MS would be methods of choice.
By fluorescence you cas use nonyl-acridine-orange (which fluoresces green).
It has big affinity for cardiolipids.
By spectrofluorimetry you standardize with NAO alone, then make a concentration curve by adding growing concentratiions of purified cardiolipids with a constant NAO concentration. In later step you could exctract mitochondria from your frozen tissue by subcellular fractionning, and then determine the concentration of cardiolipid in your mitochondria enriched fraction.
Thank you Olivier, have you any protocol for the spectrofluorimetry method?
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