Well... I decided to design some primers on miRprimer and I could achieve good results, with E>90%, R2>=0.98. So, I think that it is a good software and I'm going to use it to design my primers.
I want to design primer against a miRNA But unable to design as the 'README' instruction says that the new FASTA miRNA sequence should be typed in the input file. Does any body know how to design primers?